Italian 
English CONTRIBUTI SCIENTIFICI – Scientific Papers
Volume:
Biochimica Clinica 2020; 44(3) 232-238
Pubblicato on-line:
Marzo 6, 2020
DOI:
10.19186/BC_2020.016
Effetti della restrizione calorica sullo stress ossidativo nell’obesità: sonomiglioramenti transitori?
Effect of caloric restriction on oxidative stress in obesity: are these transient improvements?
AUTORI
1Centro Stress Ossidativo (CSOx), Vicenza
2Servizio di Igiene degli Alimenti e Nutrizione (SIAN), ULSS8 Berica, Vicenza
3Laboratorio Analisi Ospedale S. Bortolo, ULSS8 Berica, Vicenza
4Dipartimento di Medicina, Università di Verona
ABSTRACT
Effect of caloric restriction on oxidative stress in obesity: are these transient improvements?
Background: the effects of caloric restriction (CR) on oxidative stress in obesity has been previously studied using markers that not always were able to describe all the components of the oxidative-inflammatory picture.
Methods: in this study, the redox state of 20 obese was evaluated at baseline and after 30 and 60 days of CR using a complete panel of markers: the majority of them were determined using HPLC methods.
Results: before CR (V0), serum peroxides (dROMs) were very high, total antioxidant barrier (BAP) was at the lower limit of the reference interval and C-reactive protein (hsCRP) was increased; on the opposite, glutathione was well within the reference intervals in both total and reduced form. Despite the imbalance of the dROMs/BAP equilibrium, the markers of oxidative damage, such 3-nitrotyrosine (3NT) and 8-hydroxy-deoxyguanosine (8OHdG), index of a mild oxidative-inflammatory imbalance, were not particularly relevant.
After 30 days of CR (V30), in addition to the slight improvements of glucose, fructosamine and HOMA-IR, hsCRP was decreased, while BAP and total glutathione were increased, with consequent improvement of the oxidative stress-inflammatory balance (oxidative-inflammation). After 60 days of CR (V60) the improvements observed at V30 appeared to be slowing down for glucose and fructosamine, in slight inverse tendency for HOMA-IR and hsCRP, and decreasing for BAP and glutathione. The slight increase of inter-quartile range (IQR) of 3NT showed a lower counter-regulatory antioxidant response capacity, as if the ameliorative effects of CR in the first period had turned off.
Conclusion: the improvements of the oxidative-inflammatory equilibrium appear to be transient in the course of CR. The rearrangements of the gut microbiota during CR and the consequent epigenetic modulations could be responsible for this peculiar trend.