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Italian CONTRIBUTI SCIENTIFICI – Scientific Papers
Volume:
Biochimica Clinica 2014; 38(4) 302-306
Pubblicato on-line:
DOI:
Association of apolipoprotein(a) double band phenotype and elevated lipoprotein(a) concentrations in Ivorian subjects suffering from arterial hypertension and myocardial infarction
AUTORI
1Laboratory of Biochemistry, UFR Pharmaceutical and Biological Sciences, Abidjan, Côte d’Ivoire
2Laboratory of Medical Biochemistry, Institut Pasteur, Abidjan, Côte d’Ivoire
3Laboratory of Pediatric Biology and Research, CHU of Reims, France
4Laboratory of Medical Biochemistry, Faculty of Medicine, UMR CNRS/URCA No 6237, Reims, France
5Institute of Cardiology, CHU of Treichville, Abidjan, Côte d'Ivoire
6Laboratory of Biophysics and Statistics, UFR Pharmaceutical and Biological Sciences, Abidjan, Côte d’Ivoire
ABSTRACT
The purpose of this study was to assess the relationship between apolipoprotein(a) [apo(a)] isoform size and lipoprotein(a) [Lp(a)] concentrations in Ivorian subjects suffering from essential arterial hypertension (AH) and myocardial infarction (MI). The investigation concerned 91 patients: 61 with AH and no other clinically evident pathologies and 30 with a prior diagnosis of MI. 102 matched healthy subjects served as controls. Serum Lp(a) concentrations were determined by nephelometry. Apo(a) isoforms were determined using a SDS-PAGE followed by immunoblotting. Lp(a) concentrations were increased in AH and MI patients compared to controls. Apo(a) phenotyping distribution revealed that apo(a) isoform sizes ranged from 11 kringles to 35 kringles. There was no significant difference between apo(a) size in controls and patients. Patients and controls with “double band” phenotype showed higher Lp(a) concentrations than in their corresponding group with “single band” phenotype. Regarding the “single band” phenotype, no significant difference was observed in Lp(a) concentrations between patient and control groups. However, serum Lp(a) concentrations were significantly increased in AH and MI patients with “double band” phenotype. The inverse relationship between apo(a) size and Lp(a) concentrations was present in controls and patients with “double band” phenotype. Apo(a) “double band” phenotype is associated with elevated concentrations of Lp(a) in Ivorian patients with AH and MI.