English 
Italian CONTRIBUTI SCIENTIFICI – Scientific Papers
Volume:
Biochimica Clinica 2019; 43(4) 406-412
Pubblicato on-line:
July 26, 2019
DOI:
10.19186/BC_2019.041
Studio preliminare del microbiota intestinale, cutaneo e della mucosa orale in pazienti affetti da Pemfigo Volgare e Pemfigoide Bolloso
AUTORI
1Laboratorio di Diagnostica Molecolare e Genomica, Unità Operativa Complessa di Chimica, Biochimica e Biologia Molecolare, Fondazione Policlinico Universitario “A. Gemelli” IRCCS, Roma
2Laboratorio di Diagnostica Molecolare Avanzata (DIMA), Istituto Dermopatico dell'Immacolata (IDI)-IRCCS, FLMM, Roma
3Laboratorio di Oncologia Molecolare, Fondazione “Giovanni Paolo II", Università Cattolica del Sacro Cuore, Campobasso
4Prima Divisione di Dermatologia, Istituto Dermopatico dell'Immacolata (IDI)-IRCCS, FLMM, Roma
5Dipartimento di Scienze e Innovazione Tecnologica, Università degli Studi del Piemonte Orientale, Alessandria
ABSTRACT
Preliminary study of the microbiome in the gut, skin and oral mucosa of patients affected by Pemphigus Vulgaris and Bullous Pemphigoid
Introduction: the study of the human microbiome is one of the most dynamic current topics in biomedical research. A significant challenge in this field is the development of cost effective method for a robust interrogation of microbiota composition. Advances in next-generation sequencing (NGS) technologies have allowed for efficient and molecular-based analysisof microbial communities. Association between diseases and imbalance of the microbial populationsare today wellinvestigated.
Methods: Pemphigus Vulgaris (PV) and Bullous Pemphigoid (BP) are two rare autoantibody-mediated blisteringskin diseases. In this pilot study, we characterized the intestinal, cutaneous and oral mucosal microbiota compositionsin PV/BP patients, in order to evaluate the potential role of the bacterial composition in these dermatologicaldisorders. Particularly, we performed a high-throughput sequencing analysis of the V3-V4 hypervariable regions of16S rRNA for the evaluation of bacterial composition of stool, skin and oral mucosa samples in PV (n=12) and BP(n=8) patients.
Results: a similar composition of the intestinal microbiota was observed in PV and BP patients. The evaluation of skin lesions revealed a prevalence of Firmicutes phylum in both patients’ groups. In the cutaneous microbiota, we identified a significant decreased abundance of Bacteroidetes phylum compared to healthy controls.
Conclusions: the results obtained from our standardized NGS pipeline, reinforced by correlation with other clinical and biochemicalparameters, will contribute to clarify the mechanisms of these rare diseases.