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Volume:
Biochimica Clinica 2018; 42(3) 263-265
Pubblicato on-line:
Giugno 14, 2018
DOI:
10.19186/BC_2018.037
Raccomandazioni per l’ottimizzazione della fase pre-analitica per una corretta determinazione della glicemia in ambito diabetologico
AUTORI
1Laboratorio Centrale Analisi Chimico-Cliniche, Azienda Sanitaria Socio Territoriale - Spedali Civili, Brescia
2Laboratorio di Chimica Clinica ed Ematologia, Ospedale San Bortolo, Vicenza
3Dipartimento di Medicina, Università degli Studi, Padova
4Dipartimento di Medicina Clinica e Sperimentale, Sezione Diabetologia e Malattie Metaboliche, Università degli Studi, Pisa
5Laboratorio analisi chimico-cliniche e molecolari, Istituto Nazionale di Ricovero e Cura per gli Anziani (INRCA), Ancona
6Dipartimento di Fisiopatologia medico-chirurgica e dei Trapianti, Università degli Studi, Milano
ABSTRACT
Correct determination of glycemia in the management of diabetes: recommendations for the optimization of the pre-analytical phase
The time-dependent decrease of glucose in tubes after venipuncture may cause artificially lower values, if glycolysis is not appropriately inhibited by the correct anticoagulant. In this work we have extensively reviewed the current literature about the possible use of citrate buffer together with sodium EDTA and sodium fluoride. We conclude that, for screening and diagnosis of diabetes mellitus, including gestational diabetes, glucose has to be determined in plasma by using the above mentioned ternary mixture either as solid or in liquid state (in this case the correct numerical conversion factor has to be employed). For the measurement of glucose in patients with already known diabetes and following monitoring, lithium heparin tubes may be used providing that plasma separation should be rapidly performed. Alternatively, serum-separating tubes with particles promoting rapid clotting could also be employed.