English 
Italian Development of new tools for molecular diagnosis of hematologic malignancies
AUTHORS
Silvia Salmoiraghi1, Giulia Minnucci2, Giulia Amicarelli2, Pamela Zanghì1, Lara Elidi1, Marie Lorena Guinea Montalvo1, Francesca Rigo2, Elena D’Agostini2, Veronica Tettamanzi2, Manuela Giachelia2, Francesco Colotta2, Alessandro Rambaldi1,3, Orietta Spinelli1
1Hematology and Bone Marrow Transplant Unit, ASST-Ospedale Papa Giovanni XXIII, Bergamo
2DiaSorin SpA, Gerenzano (VA)
3Dipartimento Scienze Cliniche e di Comunità, University of Milan, Milano
1Hematology and Bone Marrow Transplant Unit, ASST-Ospedale Papa Giovanni XXIII, Bergamo
2DiaSorin SpA, Gerenzano (VA)
3Dipartimento Scienze Cliniche e di Comunità, University of Milan, Milano
ABSTRACT
Molecular alterations represent diagnostic and prognostic factors in hematologic diseases. Their presence can anticipate the malignancy outcome or confer responsiveness to specific treatments. Here we present the development of new assays based on an innovative technology, named loop-mediated isothermal amplification (LAMP), for the molecular detection of these alterations in a simple, fast and accurate way. These assays can identify molecular aberrations in a non polymerase chain reaction-based manner, starting directly from DNA or RNA in a single-tube close format and in a short time frame.