Risultati preliminari per la definizione dell’intervallo di riferimento per l’indoxyl solfato (indicano) nelle urine
Preliminary results for the definition of a reference interval for urine indoxyl sulfate (indican)

AUTORI

ABSTRACT

Preliminary results for the definition of a reference interval for urine indoxyl sulfate (indican).

Introduction: the term “gut dysbiosis” refers to an alteration in the composition and functions of the microbiota that causes the loss of beneficial microorganisms and species diversity with the potential growth of harmful bacteria. This condition has been related to many pathologies, both intestinals and extraintestinals. One method for evaluating the dysbiosis is the determination of urinary metabolites related to intestinal fermentative processes. Among these different molecules, indoxyl-sulfate (IS) is well studied. In this paper, the IS measurement was performed in a group of subjects in order to establish a reference interval.
Methods: the measurement was carried out by HPLC method using a  fluorescence detector on early morning urine void. The results were expressed for urine creatinine concentration.
Results: in a group of 68 subjects (35 males, 33 females), the mean IS concentration was 22.3 µmol/mmol creatinine with a standard deviation of 6.8 and a range of 8.2-36.6. The reference interval at 95%, calculated with the “robust method”, was 9.0-36.3 µmol/mmol creatinine. The 90% confidence intervals, calculated with the “boostrap method”, for the lower and upper limit of the reference interval were 6.8-11.5 and 34.0-38.6 respectively.
Conclusion: this study is  one of the first contributions to the definition of a reference interval for this metabolite in the first morning urine void.

BIBLIOGRAFIA