Serial measurements of oxidative stress markers after ozone autohemotherapy

AUTORI

ABSTRACT

Background: the ozone autohemotherapy (O3-AHT) is an alternative medical practice where an aliquot of patient’s blood is treated with an ozone-oxygen mixture (O3/O2), and reinfused to induce an hormetic effect, that is to obtain a mild oxidative stress that empowers the antioxidant response and improves the oxidative balance. The customization of the ratio O3/O2 and the amount of blood is a crucial aspect of this therapy. The measure of some biomarkers of oxidative balance may be useful to calibrate the treatment and avoid damages.
Methods: O3-AHT was performed on 9 volunteers healthy subjects, treating 150 g of blood with 30 μg/mL O3/mL O2/g. Aliquots of blood and urine were collected before therapy (t0), and 4, 8, 24 and 48 hours (t4-t48) after reinfusion to measure the derivates of reactive oxygen metabolites (dROMs), the biological antioxidant potential (BAP), the single components of antioxidant barrier coenzyme Q10, and glutathione in both total (GSH+GSSG) and reduced (GSH) forms, the oxidative biomarkers homocysteine (Hcy), 8-hydroxy-deoxyguanosine (8-OHdG) and 2-deoxyguanosine (2-dG) and the nitrative biomarker 3-nitrotyrosine (3-NT).
Results: within the first 4-8 hours after O3-AHT, as dROMs increased, the median of GSH had an initial considerable decrease (-20% at t8 versus t0) followed by a final net increase (+12% at t48). In a similar way, 3-NT had an initial decrease (-19% at t4) followed by a remarkable increase at t8 and t24 (+28% and +57% respectively) and a subsequent important decrease at t48 (-44%), which led to final nitration levels lower than the one observed at t0.
Conclusions: When used in correct concentrations, O3 induces a moderate oxidative stress which initially consumes antioxidant species and produces nitrative damages. Within 24-48 hours however, the beginning of a virtuous counter-regulatory response that increases the reducing power (particularly GSH levels) and decreases molecular damages, improving the redox balance, can be observed. Serial measurements of GSH and 3-NT, allow to monitor that the induced oxidative stress causes only moderate and transient damages, and help the physician to choose the more effective O3 therapeutic concentrations.

Background: the ozone autohemotherapy (O3-AHT) is an alternative medical practice where an aliquot of patient’s blood is treated with an ozone-oxygen mixture (O3/O2), and reinfused to induce an hormetic effect, that is to obtain a mild oxidative stress that empowers the antioxidant response and improves the oxidative balance. The customization of the ratio O3/O2 and the amount of blood is a crucial aspect of this therapy. The measure of some biomarkers of oxidative balance may be useful to calibrate the treatment and avoid damages.
Methods: O3-AHT was performed on 9 volunteers healthy subjects, treating 150 g of blood with 30 μg/mL O3/mL O2/g. Aliquots of blood and urine were collected before therapy (t0), and 4, 8, 24 and 48 hours (t4-t48) after reinfusion to measure the derivates of reactive oxygen metabolites (dROMs), the biological antioxidant potential (BAP), the single components of antioxidant barrier coenzyme Q10, and glutathione in both total (GSH+GSSG) and reduced (GSH) forms, the oxidative biomarkers homocysteine (Hcy), 8-hydroxy-deoxyguanosine (8-OHdG) and 2-deoxyguanosine (2-dG) and the nitrative biomarker 3-nitrotyrosine (3-NT).
Results: within the first 4-8 hours after O3-AHT, as dROMs increased, the median of GSH had an initial considerable decrease (-20% at t8 versus t0) followed by a final net increase (+12% at t48). In a similar way, 3-NT had an initial decrease (-19% at t4) followed by a remarkable increase at t8 and t24 (+28% and +57% respectively) and a subsequent important decrease at t48 (-44%), which led to final nitration levels lower than the one observed at t0.
Conclusions: When used in correct concentrations, O3 induces a moderate oxidative stress which initially consumes antioxidant species and produces nitrative damages. Within 24-48 hours however, the beginning of a virtuous counter-regulatory response that increases the reducing power (particularly GSH levels) and decreases molecular damages, improving the redox balance, can be observed. Serial measurements of GSH and 3-NT, allow to monitor that the induced oxidative stress causes only moderate and transient damages, and help the physician to choose the more effective O3 therapeutic concentrations.

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