English 
Italian Determinazione degli intervalli di riferimento dell’esame emocromocitometrico eseguito con analizzatore Sysmex XN 9000
AUTORI
Alberto Crippa1, Cosimo Ottomano2, Giuseppe Lippi2
1Laboratorio di Analisi Chimico Cliniche, Azienda Ospedaliera Papa Giovanni XXIII, Bergamo
2UO Diagnostica Ematochimica, Azienda Ospedaliero-Universitaria di Parma
ABSTRACT
We aimed to define reference intervals for complete blood count in 240 apparently healthy Italian adults (120 males and 120 females) using Sysmex XN 9000 platform, as recommended by international standards. The recruitment criteria for reference individuals were based on negative anamnesis and physiological serum concentrations of glucose, creatinine, transaminases, ferritin and C-reactive protein. The results were comparable to those previously generated on different European populations. Interesting results were found for some research parameters, for which limited information was available in Italian populations so far. With regard to reticulocyte-related parameters, a significant gender difference was found for reticulocyte hemoglobin, highly fluorescent reticulocyte fraction and delta-He, which represents the difference in hemoglobin content between reticulocytes and erythrocytes. The results also support the reference interval of immature platelet fraction observed in previous studies, except for the absolute value for which larger range and higher values were found. For leukocyte morphological and functional parameters, we were able to define reference intervals in the whole study population as well as in male and female subgroups, since gender-related differences were observed for some parameters.